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[Epigentek] Global DNA Methylation (5-mC) ELISA Easy Kit 부경에스엠 2016.03.14
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 EPIGENTEK

Global DNA Methylation in Just One-Step and 2 Hours

Measuring Global 5-mC just got better with our new MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric). This kit allows you to quantify global DNA methylation status by specifically measuring levels of 5-methylcytosine (5-mC) in a simplified, "one-step" ELISA-like format. 

  • Fast - Reduced steps allow you to finish in only 2 hours
  • Robust - Improved kit has a greater "signal window" with reduced variation between replicates
  • Convenient - Inherently low background noise without the need for DNA denaturation and plate blocking steps
  • Sensitive - Detection limit can be as low as 0.05% methylated DNA from 100 ng of input DNA 
  • Specific - No cross-reactivity to unmethylated or hydroxymethylated cytosine 
  • Universal - Included positive and negative controls allow for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA  
  • Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis
  • Flexible - Strip-well microplate format for manual or high throughput analysis
? View Kit Now

** MethylFlash™ Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit available soon!  **
  
Schematic procedure for the MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit.

Accurate quantification of 5-mC content of various DNA samples from different species using the MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit. The results are closely correlated with those obtained by HPLC-MS.

So what's the difference between this new kit (#P-1030) and the previous MethylFlash kit (#P-1034)?


The MethylFlash Easy kit, while still retaining the many advantageous features of the previous kit, utilizes a one-step ELISA reaction to eliminate the need for multiple sample and antibody binding steps. The protocol of the new kit is simplified, halving the procedure time versus the previous kit. Some adjustments were made to also reduce the CV% between wells. The greater "signal window" of the kit, along with its adjusted calculation formulas, makes it very accurate. In a nutshell, MethylFlash Easy is faster, easier, more robust, and more accurate.

 

　 Cat. #P-1034
MethylFlash™
Methylated DNA 5-mC Quantification Kit (Colorimetric)		 new #P-1030
MethylFlash™
Global DNA Methylation (5-mC)
ELISA Easy Kit (Colorimetric)
Assay Principle Direct ELISA Direct ELISA
Format 96-well plate 96-well plate
Sensitivity as low as 0.2 ng  as low as 0.05 ng
(< 0.05 % from 100ng of input DNA)
Protocol Time  < 4 hr < 2hr
Standard Control NC: 0 % 5-mC
PC: 50 % 5-mC
6 concentration point
(0, 0.5, 1, 2, 5, 10 ng/ul)		 NC: 0 % 5-mC
PC: 5 % 5-mC
6 concentration point
(0, 0.1, 0.2, 0.5, 1, 2, 5 %)
Input Amount
(Optimal input amonut)		 50 ng - 200 ng
(100 ng)		 20 ng - 200 ng
(100 ng)



 

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