Did you know you can use Stellaris? RNA FISH to study chromosomes? This month, there have been two new publications citing Stellaris, one of which focuses on spatial localization of genes across chromosomes. Specifically, noncoding RNAs (ncRNAs) have recently been discovered to localize to or originate from chromosomal elements including centromeres, pericentromeres, and telomeres opening a new area of experimental investigation for RNA FISH.
The first highlighted publication is a nice review of repeat RNAs in chromosome research in Methods in Enzymology by D. Qu?net et al., Identifying Centromeric RNAs Involved in Histone Dynamics In Vivo. This work examines the new paradigm suggesting that ncRNAs may participate in regulating processes such as transcription-mediated nucleosome assembly, chromatin fiber stabilization, and recruitment of chromatin-binding factors. Our second paper in focus today is Topologically associated domains enriched for lineage-specific genes reveal expression-dependent nuclear topologies during myogenesis by D. Neems et al. and published in PNAS PLUS. Here, the relationship between linear and 3D organization of gene regulation during myogenesis is examined and linked to transcription regulation and cell division. Visit our Citation Center to learn more about these and the hundreds of other new publications featured there.
If you wish to learn more about Stellaris RNA FISH, please view our Comprehensive Information Page or contact me with questions.
Best wishes,
Jessica Kaplunov, Ph.D
Product Manager
LGC Biosearch Technologies
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