 | 행사 기간 2018년 9월 1일 ~ 12월 31일 |
Cell Fusion & Cell Transfection Kit GenomeONE 20% 할인 ü HVJ-E(Hemagglutinating virus of Japan Envelope, inactivated Sendai virus)를 이용
ü Membrane fusion 방식
ü Transfection 적용 - Plasmid DNAs, siRNAs, oligonucleotides, proteins 등 | | | | GenomeONE™ – Neo Ex
HVJ-E Transfection Kit
for introduction of DNA, siRNA and Protein | | • Applicable to in vitro and in vivo transfection experiments • Transfection mediated by membrane fusion completely different from lipid-based reagents • Unlikely to undergo lysosomal degradation, enabling high efficiency of transfection • Performance demonstrated in more than 200 published papers | | |  | | | | Inhibition of spindle orientation through Eg5 knock down (HT1080 cell/red: DNA, green: α-tubulin). | | | | | |
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| | | | | GenomeONE™ – CF Ex
HVJ-E Cell Fusion Kit | | | | | |  Cell fusion with GenomONE-CF EX (HVJ-E Cell Fusion Kit) (time course of 67 minutes) BHK-21 cells labeled with red and green fluorescent dyes incubated at 37℃ in the presence of HVJ-E and observed under a confocal laser scanning microscope. | | | | | |  HVJ-E: Hemagglutinating virus of Japan (Sendai virus） envelope | • For preparation of hybridomas (monoclonal antibodies) • For research on development, differentiation, and breeding
(transplantation and replacement of nuclei) • For research on regenerative medicine and cytotherapy • For research on anti-cancer vaccination and cancer immunology | | | | | |
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ü HVJ-E는 완벽하게 불활성화 되어 있고, Sendai virus envelope protein의 cell membrane-fusing 능력만을
가지고 있습니다. ü HN protein이 cell membrane의 receptor를 인식하여 흡수하고, F protein에 의해 membrane fusion이
일어나게 됩니다. ü 전통적인 virus를 이용한 transfection 방법은 endocytosis를 통하여 이루어 지기 때문에 lysosome에 의한
transffered DNA의 분해가 일어나지만, HVJ-E vector는 이에 대한 저항성이 있기 때문에 specific DNA
전달이 더 용이하며, 높은 yield로 유전자 발현 효율도 높습니다. | 1. siRNA transfection
in vitro:
Primary T cell (Human peripheral blood)
Primary cardiac myocyte (Rat cardiac myocyte)
Differentiated C2C12 (Mouse myoblast)
monkey ES cells
Min6 (Mouse pancreatic (BETA) cell)
U937 (Human myelomonocytic cell)
in vivo:
Intradermally transplanted human cervix cancer /HeLa (SCID mouse)
Submandibular gland (rat) 2. Protein delivery
in vivo:
Primary macrophage (C3H mouse peritoneal resident)
Swiss 3T3 cell (Mouse embryonic fibroblasts)
in vivo:
Nucleus tractus solitarius (Rat brain) 3. Oligo DNA transfection
in vitro:
Primary HDMECs (Human dermal microvascular endothelial cell)
Primary CD34+ cell (Human blood)
in vivo:
Uterus (Mouse)
Skin, ear lobe(Mouse)
Lung (Mouse) 4. Plasmid DNA transfection
in vivo:
Uterus(Mouse)
Palatal periodontal tissue (Rat)
Myocardium (Rat heart)
Lung (neonatal porcine)
Subcutaneously transplanted human colon cancer /LoVo (nude mouse) |
학술문의 T. 031-728-3231 E. technical@dawinbio.com |
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